Suche
Lesesoftware
Info / Kontakt
Pericyte Biology - Novel Concepts
von: Alexander Birbrair
Springer-Verlag, 2018
ISBN: 9783030026011 , 172 Seiten
Format: PDF, Online Lesen
Kopierschutz: Wasserzeichen
Preis: 96,29 EUR
eBook anfordern 
Preface
5
Contents
7
Contributors
9
Chapter 1: Pericyte Biology: Development, Homeostasis, and Disease
11
References
12
Chapter 2: The NG2 Proteoglycan in Pericyte Biology
14
Introduction
15
NG2 as a Pericyte Marker
15
Mechanisms of NG2 Action
18
NG2-Dependent Aspects of Pericyte Function
18
Future Prospects
23
References
24
Chapter 3: Pericytes for Therapeutic Bone Repair
29
Introduction
30
Identification and Purification of Perivascular Cells for Bone Repair
31
Pericytes and Ectopic Bone Formation
32
Pericytes in Calvarial Defect Regeneration
33
Pericytes in Spinal Fusion
34
Pericytes for Non-union Fracture Healing
34
Discussion
35
References
37
Chapter 4: Pericyte Biology in Zebrafish
41
Early Development of the Zebrafish Vascular System
42
Zebrafish as a Model for Studying Blood Vessel Development
42
Developmental Origin of Pericytes in Zebrafish
43
Determining the Origin of Cerebral Pericytes Using Mutants and Morphants
43
Lineage Tracing of Cerebral Pericytes Using Transgenic Zebrafish
43
Developmental Origins of Trunk Pericytes
44
Determining the Origin of Trunk Pericytes Using Mutants and Morphants
44
Lineage Tracing of Trunk Pericytes Using Transgenic Zebrafish
45
Spatiotemporal Pericyte Marker Expression in the Developing Zebrafish Brain
45
Spatiotemporal Pericyte Marker Expression in the Developing Trunk Vasculature
47
Pericyte Recruitment to the Endothelium
49
Haemodynamic Forces Are Required to Recruit Pericytes to the Endothelium
49
Mural Cell Migration After Recruitment
50
Signalling Pathways Involved in Mural Cell Development, Differentiation and Recruitment
50
Sonic Hedgehog Signalling Is Required for Pericyte Development
50
TGF?/BMP Is Involved in Pericyte Development
51
Notch Signalling Regulates Mural Cell Development and Recruitment
51
PDGF Signalling Recruits Mural Cells to Vessels
52
Pericytes in Zebrafish Organs
52
Pericytes in the Zebrafish Eye
52
Mural Cells in the Visceral System
53
Mural Cell Expression in the Developing Heart
53
Zebrafish Models of Disease Involving Pericytes
53
Notch3 and CADASIL
53
Foxc1 and Foxf2 Are Involved in Mural Cell Development
54
The Role of Pericytes in Regulating Vascular Tone
54
A Novel Population of Perivascular Mural Cells in the Zebrafish Brain
55
Future Perspectives on Zebrafish Pericyte Research
56
References
56
Chapter 5: The Microvascular Pericyte: Approaches to Isolation, Characterization, and Cultivation
60
Introduction
60
Cell Isolation and Separation: Basic Considerations
61
Preparing for Pericyte Isolation
62
Digestion/Homogenization of Solid Tissues for Preparation of Single Cell Homogenates
62
Isolation of Pericytes from Single Cell Homogenates
63
Immuno-panning
63
MACS
64
Fluorescence-Activated Cell Sorting (FACS)
64
The Subculture of Pericytes from Purified Preparations of Cerebral Microvessels
65
Generation of Pericyte Lines
66
References
69
Chapter 6: Pericytes in Veterinary Species: Prospective Isolation, Characterization and Tissue Regeneration Potential
73
Introduction
74
Prospective Isolation of Pericytes from Tissues
75
Pericytes in Veterinary Species
75
Horse
76
Dog
77
Sheep
78
Pig
78
Conclusions and Future Directions
79
References
80
Chapter 7: Pericytes in the Human Vocal Fold Mucosa
84
Introduction
85
Vascular Network of the Human Vocal Fold Mucosa as a Vibrating Tissue
85
Microstructure of the Blood Vessels and Pericytes in the Human Vocal Fold Mucosa
86
Arterioles
86
Capillaries
88
Venules
89
Development of the Blood Vessels and Pericytes in the Human Vocal Fold Mucosa
90
Physiological Significances of the Vascular Network and Pericytes in the Human Vocal Fold Mucosa
91
Vascular Network in the Human Vocal Fold Mucosa
91
Pericytes in the Human Vocal Fold Mucosa
92
Deseases Related to the Vascular Network and Pericytes in the Human Vocal Fold Mucosa
93
Reinke’s Edema
93
Hemorrhage in Reinke’s Space and Vocal Fold Polyps
95
Microvascular Leisions of the Vocal Fold
95
Vocal Fold Tissue Injury
97
References
98
Chapter 8: Ca2+ Signalling in Pericytes
99
Introduction
100
Topology and Morphology of Pericytes
100
Contractility
102
Electrical Activity, Ion Currents and Channels
103
Ca2+ Signalling and Excitation-Contraction Coupling
103
VGCC-Dependent Ca2+ Entry Induced by Membrane Depolarization
104
VGCC-Dependent Intercellular Ca2+ Signalling
104
Synchronized Ca2+ Oscillations in Pericytes of Postcapillary Venules
105
Propagating Intercellular Ca2+ Waves in Ureteric Microvessels
105
Ca2+ Signalling Induced by GPCR Agonists
106
IP3R-Mediated Intracellular Ca2+ Waves and Oscillations
106
Voltage-Independent Ca2+ Entry
108
Effects of Metabolic Factors
108
Summary and Conclusions
109
References
109
Chapter 9: Pericytes Derived from Human Pluripotent Stem Cells
114
Introduction
114
Human Pluripotent Stem Cell (hPSC) Sources
115
Pericytes Derived from hPSC
116
Verification of Phenotype and In Vivo Functionality
121
Tissue Specificity and Differentiation of Mural Cell Subtypes
123
Future Directions
124
References
125
Chapter 10: Pericytes in Tissue Engineering
128
Introduction
128
Pericytes in Tissue Engineering
130
Pericytes in Bone Tissue Engineering
131
Pericytes in Cartilage Tissue Engineering
132
Pericytes in Dermal Tissue Engineering
132
Pericytes in Vascular Tissue Engineering
133
Pericytes in Cardiac Tissue Engineering
134
Pericytes in Blood Tissue Engineering
135
Future Perspective
136
References
136
Chapter 11: Pericyte Secretome
141
Introduction
143
The Pericyte Secretome Under Basal Conditions
145
The Secretome of Pericytes Under Inflammatory Conditions
146
LPS
146
IL1-?, TNF-?
152
IFN-?
152
Exposure of Brain Pericytes to Specific Pathogens (CMV, HIV, JE)
153
The Secretome of Pericytes Upon Exposure to High Glucose
154
The Secretome of Pericytes and Control of the Tumor Microenvironment
154
The Secretome of Pericytes in Angiogenesis
155
The Secretome of Pericytes Under Hypoxic Conditions
156
The Secretome of Pericytes After Other Pathological Stimuli
157
Exposure to NO
157
Exposure to Advanced Glycation Products
157
Exposure to TGF-?
158
Microvesicles and Pericytes
158
The Role of the Pericyte Secretome -Regenerative Potential
159
Future Trends
160
References
161
Correction to: The Microvascular Pericyte: Approaches to Isolation, Characterization, and Cultivation
166
Correction to: Chapter 5 in: A. Birbrair (ed.), Pericyte Biology - Novel Concepts, Advances in Experimental Medicine and Biology 1109, https://doi.org/10.1007/978-3-030-02601-1_5
166
Index
167